Brain Mind Robotics |
"Silicon Valley" (Palo Alto - San Jose ) California
We Are Building a Robotic Bio-Computer, Based on the Functional Neuroanatomy of the Human Brain, and Which Can Speak, Reason, Read, Understand Language, Experience Self-Consciousness and Human Emotions, Think Creatively, and Physically Interact with the Environment.
We Will be Hiring Stem Cell Neurobiologists with an Extensive Background in Bioinformatics, Physics, Mathematics, Computer Science, Statistics, Biology, or Engineering, or related fields, and strong scientific background and proven accomplishments in neural stem cells, 3D cellular culturing and the use of microfluidic chambers, and the generation, maintenance, differentiation and characterization of 3D cellular models derived from primary human or iPSCs.
STAGE 2: The Generation of Neural Interfaces and the creation of a stand-alone unit which is programmed to "reflexively" respond to simple visual and auditory stimuli, and to "reflexively" move the "eyes", turn the "head", open and close the "mouth" and its "hands", and make sucking, chewing, swallowing, swimming, and leg-lifting stepping movements, and to raise its "arms" and touch its "mouth" and "face."
Stem Cell Neurobiologist--Robotics--Qualifications
• Extensive hands-on experience in working with stem cells, developing complex functional assays, 3D cellular modeling and the use of microfluidic devices is preferred.
• Experience with genomics, epigenomics, aging epigenome, neuron stem cell, machine learning, or cloud computation is a plus.
• Ability to design and conduct novel cell biology and stem cell research to create neurons and neural networks which will interface with a biocomputer.
• Experience in planning and executing large in vivo experiments and applying a variety of methods in molecular and cell biology, biochemistry, and cell culture as well as 'omics' techniques including RNA sequencing, and high-content imaging.
• Experience developing, generating and establishing robust protocols to grow 3D cellular models or organoids (brain) from primary human or iPSCs.
• Experience in establishing functional assays using various techniques including imaging, FACS, in vitro electrophysiology, etc.
• Experience with iPS cell growth and differentiation into various nerve cell lineages, iPSC-derived cell-based assay systems and microfluidic devices is preferred.
• Proficiency in growing, maintaining, characterizing human iPSC or ESC.
• Proficiency in generating human iPSC or derivative cell types (e.g. NPC, neurons).
• Proficiency in molecular biology techniques and previous experience with PCR, genotyping, and/or DNA extraction a plus.
• -Perform standard laboratory procedures with Human Embryonic Stem Cells (hESC), neuronal precursor cells (NPCs), induced neurons (iNeurons), and mature neurons (mNeurons), and brain organoids.
• Assist in the general growth and maintenance of fibroblast and stem cell lines (adult and embryonic derived).
• Assist in Gene manipulation of these stem cells with, CRISPR targeting, shRNA silencing or overexpression using transfection or lentivirus transduction
• Assist in the characterization of resultant phenotypes by molecular and imaging techniques.
• Experience in biology techniques to isolate RNA and quantify gene expression.
• Assist in the generation of transgene constructs, PCR and RNA isolation) and microscopy as well as basic molecular and cellular biology techniques.
• Assist in cell transfection, genomic screening, cloning, gene expression, immunocytochemistry, tissue culture.
• Ability to independently perform tissue culture of primary cells, preparation of lentiviruses and viral infection techniques, maintain tissue culture facility stocks and inventory.
• Ability to perform cell culture electroporation in stem cells and independently perform routine biology and biology techniques including next generation sequencing techniques.
• Grow and maintain stocks of patient derived iPSC and maintain tracking records.
• Use cutting edge methods (e.g. CRISPR/Cas9, Zfn or other) to create new human iPSC with targeted mutations in disease genes or modifier genes.
• Use different inductive protocols to generate specific cell types (e.g. neuronal cells) from iPSC.
• Assist in developing primary neurons and iPS cell-derived neurons.
• Assist in developing assays to validate the activity and targets of cultured neurons generated from stem cells.
• Evaluate identified and novel small molecule activity and properties.
• Ability to generate and maintain embryonic primary neurons and assist in grafting these neurons to robotic visual, auditory, tactile, and motor platforms.
• Ability to grow and maintain stocks of iPSC-derivative cell types that are progenitor cells (e.g. neuronal progenitor cell populations)
• Experience performing standard molecular biological techniques, including PCR, DNA extraction, genotyping, Southern blotting and qPCR.
• Ability to run quality control assays and other sample preparation techniques on iPSC (NPC etc.) in preparation of downstream genetic analysis.
• Experience performing high content image-based assays with iPSC and derivative cell types (e.g. cell painting).
• Proficiency in basic lab techniques (Mammalian cell culture, ELISA, Western Blot, rtPCR) and principles of cell biology, biochemistry or molecular biology required.
• Proficiency in rodent embryonic brain dissection and harvesting primary neurons techniques is highly desirable.
• Ability to analyze results of high content assays, maintain image data files and extracted features.
• Ability to contribute to troubleshooting experiments and developing new laboratory processes and technologies relevant to iPSC generation, characterization and analysis.
• Demonstrated ability to perform molecular biology techniques including PCR, DNA preparation/extraction, gel electrophoresis, sterile technique.
• Experience performing laboratory procedures with Human Embryonic Stem Cells (hESC), neuronal precursor cells (NPCs) and brain organoids.
• Proven experience maintaining human embryonic stem cells, neuronal precursor cells, & hESC derived motor neurons in undifferentiated state.
• Demonstrated experience generating motor neurons.
• Demonstrated experience working with DNA, RNA.
• Ability to develop and apply bioinformatic and biostatistic algorithms to process and analyze multiple types of high-throughput sequencing data, like ChIP-Seq, RNA-Seq and Bisulfite-Seq data.
• Knowledge of and/or previous experience with mitochondrial biology is a plus.
• Previous hands-on experience in the culture and differentiation of pluripotent stem cells (PSCs) is a plus.